| 29/08/08 | Vitesse | 0-4 | Groningen De Roover 10, Van de Laak 68(p), Berg 73(p), Levchenko 78 |
| 30/08/08 | Utrecht Uiterloo 90 | 1-5 | PSV Afellay 42, 49, Amrabat 45, Koevermans 45, 77 |
| 30/08/08 | Willem II Demouge 47, Akgun 65 | 2-1 | Ajax Sulejmani 23 |
| 30/08/08 | Roda Oper 84 | 1-1 | Twente Elia 56 |
| 30/08/08 | NEC Worm 77, Otten 86 | 2-0 | De Graafschap |
| 31/08/08 | Heracles Douglas 37, Klavan 59, Bridji 90 | 3-1 | Feyenoord Andre Bahia 1 |
| 31/08/08 | Volendam Tuyp 65, 90 | 2-3 | Heerenveen Grindheim 43, Elyounossi 68, Pranjic 87 |
| 31/08/08 | Sparta John 4, Rose 60 | 2-5 | Den Haag Horvath 16, Cornelisse 19(p), Knopper 34, 69, Caracciolo 89 |
| 31/08/08 | AZ El Hamdaoui 39 | 1-2 | NAC Amoah 24, 55 |
